#GSB1004
Recombinant Protein A Beads
1mL
Catalog number
Unit size
List price(CNY)
1
GSB1004
1mL
210
Description
Recombinant Protein A beads is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein A ligand is coupled to 6% highly cross-linked agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A beads is greater than 30 mg rabbit IgG/ml settled beads. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.
Key features
Species: Recombinant Protein A expressed in E.coli.
Storage: Store at 4°C, Do NOT freeze. 1mL settled beads (2 ml 50% slurry)
Note: High temperature heating is not recommended. The agarose melts above 65°C.
Characteristics:
|
Ligand |
Recombinant Streptococcal protein A expressed in E. coli |
|
Number of IgG binding sites per ligand |
5 |
|
MW of ligand |
Approximately 34 kDa |
|
PI of ligand |
5.17 |
|
Degree of substitution |
Approximately 4 mg protein A/mL wet gel |
|
Static binding capacity |
>30 mg IgG/mL wet gel |
|
Matrix spherical |
agarose, 6% cross-linked |
|
Average particle size |
90 μm (45-165 μm) |
|
Storage solution |
20% ethanol |
|
Storage & Stability |
12 months when stored unopened at 2-8℃ |
Application data:
Figure1 Lane1: human serum; Lane2:Flow through; Lane3: wash; Lane4: elute;
Figure2 Lane1: human serum; Lane2: Flow through; Lane3: wash; Lane4: elute;
Figure 1 shows purification results of human serum by rProtein A beads of competitor. Figure 2 shows purification results of human serum by rProtein A beads of ZonHon Biopharma.
