#GSB1005
Recombinant Protein G Beads
1mL
Catalog number
Unit size
List price(CNY)
1
GSB1005
1mL
263
Description
Recombinant Protein G beads is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. The recombinant protein G ligand is coupled to 6% highly cross-linked agarose. The static binding capacity of Protein G beads is over 30 mg IgG/ml wet gel. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA
Key features
Species: Recombinant Protein G expressed in E.coli.
Storage:Store at 4°C, Do NOT freeze. 1mL settled beads (2 mL 50% slurry)
Note: High temperature heating is not recommended. The agarose melts above 65°C.
Characteristics:
|
Ligand |
Recombinant Streptococcal protein G expressed in E. coli |
|
Number of IgG binding sites per ligand |
3 |
|
MW of ligand |
Approximately 22 kDa |
|
PI of ligand |
4.69 |
|
Degree of substitution |
Approximately 4 mg protein A/mL wet gel |
|
Static binding capacity |
>30 mg IgG/mL wet gel |
|
Matrix spherical |
agarose, 6% cross-linked |
|
Average particle size |
90 μm (45-165 μm) |
|
Storage solution |
20% ethanol |
|
Storage & Stability |
12 months when stored unopened at 2-8℃ |
Application data:
Figure1 Lane1: human serum; Lane2:Flow through; Lane3: wash; Lane4: elute;
Figure2 Lane1: human serum; Lane2: Flow through; Lane3: wash; Lane4: elute;
Figure 1 shows purification results of human serum by rProtein G beads of competitor. Figure 2 shows purification results of human serum by rProtein G beads of ZonHon Biopharma.
