rProtein G resin

#GSB1005

Recombinant Protein G Beads

1mL

 

Catalog number

Unit size

List price(CNY)

1

GSB1005

1mL

250 

Description

Recombinant Protein G beads is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. The recombinant protein G ligand is coupled to 6% highly cross-linked agarose. The static binding capacity of Protein G beads is over 30 mg IgG/ml wet gel. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
    Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA

Key features

Species: Recombinant Protein G expressed in E.coli.

Storage:Store at 4°C, Do NOT freeze. 1mL settled beads (2 mL 50% slurry)

Note: High temperature heating is not recommended. The agarose melts above 65°C.

Characteristics:

Ligand

Recombinant Streptococcal protein G expressed in E. coli

Number of IgG binding sites per ligand

3

MW of ligand

Approximately 22 kDa

PI of ligand

4.69

Degree of substitution

Approximately 4 mg protein A/mL wet gel

Static binding capacity

>30 mg IgG/mL wet gel

Matrix spherical

agarose, 6% cross-linked

Average particle size

90 μm (45-165 μm)

Storage solution

20% ethanol

Storage & Stability

12 months when stored unopened at 2-8

Application data:


Figure1 Lane1: human serum; Lane2:Flow through; Lane3: wash; Lane4: elute;


Figure2 Lane1: human serum; Lane2: Flow through; Lane3: wash; Lane4: elute;

Figure 1 shows purification results of human serum by rProtein G beads of competitor. Figure 2 shows purification results of human serum by rProtein G beads of ZonHon Biopharma.