GSB5003

786-O

Organism: Homo sapiens, human/ Tissue: kidney / Disease: renal cell adenocarcinoma

 

Catalog number

Unit size

List price(CNY)

1

GSB5003

-

1000

General information

Product Format: Culturing in 25cm2 Flask

Morphology: epithelial

Culture Properties: adherent

Biosafety Level: 1

Gender: male

Applications: This cell line is suitable as a transfection host.

Characteristics

Karyotype: hypertriploid; Y was present in 60% the cells examined

Tumorigenic: Yes

Culture method

Complete Growth Medium

The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing:

Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks are recommended for subculturing this product.

1.      Remove and discard culture medium.

2.      Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.      Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.      Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5.      Add appropriate aliquots of the cell suspension to new culture vessels.

6.      Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended

Medium Renewal:  Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

References

Williams RD, et al. In vitro cultivation of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed: 1010528

Williams RD, et al. In vitro cultivation of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102

Thiede MA, et al. Human renal carcinoma expresses two messages encoding a parathyroid hormone-like peptide: evidence for the alternative splicing of a single- copy gene. Proc. Natl. Acad. Sci. USA 85: 4605-4609, 1988. PubMed: 3290897