Organism: Homo sapiens, human/ Tissue: colon; derived from metastatic site: left supraclavicular region / Disease: Dukes' type C, grade IV, colorectal adenocarcinoma


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General information                                                                      

Product Format: Culturing in 25cm2 Flask

Morphology: epithelial

Culture Properties: adherent

Biosafety Level: 1

Gender: male

Applications:This cell line is suitable as a transfection host.



The stemline chromosome number is hyperdiploid with the 2S component occurring at about 2.7% and 3 marker chromosomes were common to all S metaphases. Karyotypes were generally homogeneous and stable.

Tumorigenic: Yes


Culture method

Complete Growth Medium

The base medium for this cell line is F-12K Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


Volumes are given for a 75 cm2 flask. proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.      Remove and discard culture medium.

2.      Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.      Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.      Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5.      Add appropriate aliquots of the cell suspension to new culture vessels.

6.      Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended

Medium Renewal:  2 to 3 times per week


Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C


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Drewinko B, et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905

Drewinko B, et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465

Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538