A-375 [A375]

Organism: Homo sapiens, human/ Tissue: skin / Disease: malignant melanoma


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General information                                                                      

Product Format: Culturing in 25cm2 Flask

Morphology: epithelial

Culture Properties:adherent

Biosafety Level: 1


Applications: This cell line is suitable as a transfection host.



 It is a hypotriploid with a modal number of 62 chromosomes. There are 9 marker chromosomes that are commonly found in each cell, and normal N2, N6, and N22 are present at one copy per cell.

Tumorigenic: Yes


Culture method

Complete Growth Medium:

The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


Volumes are given for a 75 cm2 flask. Corning® T-75 flasks are recommended for subculturing this product. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

1.      Remove and discard culture medium.

2.      Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.      Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.      Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5.      Add appropriate aliquots of the cell suspension to new culture vessels.

6.      Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal:  Every 2 to 3 days


Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758

Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033

Cormier JN, et al. Heterogeneous expression of melanoma-associated antigens and HLA-A2 in metastatic melanoma in vivo. Int. J. Cancer 75: 517-524, 1998. PubMed: 9466650

Sharma SD, et al. Melanotropic peptide-conjugated beads for microscopic visualization and characterization of melanoma melanotropin receptors. Proc. Natl. Acad. Sci. USA 93: 13715-13720, 1996. PubMed: 8943000