HLF

＃GSB5008

HLF

Organism: Homo sapiens, human/ Tissue: liver, gallbladder / Disease: hepatoma, non-differentiated

	Catalog number	Unit size	List price(CNY)
1	GSB5008	-	1050

General information                                                                      

Product Format: Culturing in 25cm² Flask

Culture Properties: adherent

Biosafety Level: 1

Gender: male

Applications: This cell line is suitable as a transfection host.

Characteristics

Genetics: hepatoma, non-differentiated, age 68, male

Tumorigenic: Yes

Images:

Culture method

Complete Growth Medium: Dulbecco's modified Eagle's medium with with 5% fetal bovine serum.

pasmethod:Cells are rinsed with PBS(-) and treat with 0.2% trypsin and 0.02% EDTA at room temperature.

Pascellno: 1.5 x 10⁴ cells/sq.cm

Medium Renewal:  Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

CO₂conc: 5%

References

Establishment and some biological characteristics of human hepatoma cell lines.

Dor I,Namba M,Sato J

Gan. 1975 Aug;66(4):385-92