#GSB5008
HLF
Organism: Homo sapiens, human/ Tissue: liver, gallbladder / Disease: hepatoma, non-differentiated
Catalog number
Unit size
List price(CNY)
1
GSB5008
-
1050
General information
Product Format: Culturing in 25cm2 Flask
Culture Properties: adherent
Biosafety Level: 1
Gender: male
Applications: This cell line is suitable as a transfection host.
Characteristics
Genetics: hepatoma, non-differentiated, age 68, male
Tumorigenic: Yes
Images:
Culture method
Complete Growth Medium: Dulbecco's modified Eagle's medium with with 5% fetal bovine serum.
pasmethod:Cells are rinsed with PBS(-) and treat with 0.2% trypsin and 0.02% EDTA at room temperature.
Pascellno: 1.5 x 104 cells/sq.cm
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
CO2conc: 5%
References
Establishment and some biological characteristics of human hepatoma cell lines.
Dor I,Namba M,Sato J
Gan. 1975 Aug;66(4):385-92