＃GSB0001
Anti-His, McAb, mouse
50μg/100μg/200μg/500μg/1000μg
Catalog number
Unit size
List price(CNY)
1
GSB0001-50
50μg
1700
2
GSB0001-100
100μg
3200
3
GSB0001-200
200μg
6000
4
GSB0001-500
500μg
12000
5
GSB0001-1000
1000μg
20000
Description
GSB0001 specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, such as ELISA, Western blot and Dot blot.
As an immunogen, 6×His-tag is poorly immunogenic, so it needs to be conjugated to KLH or some other carriers. GSB0001 is produced from the hybridoma which is fusion of Sp2/0-Ag14 mouse myeloma and B-lymphocytes obtained from mouse. After hundreds of selection cycles, our researchers successfully isolated an antibody against His-tag with high specificity and sensitivity.
Key features
Source:
This monoclonal antibody GSB0001 is produced by mice immunizing with synthetic peptide HHHHHH coupled to KLH.
Isotype: Mouse IgG
Concentration: 1mg/mL
Applications: ELISA、Western blot、Dot blot and other kinds of immunoassays
Storage:
This antibody is stable in frozen form if stored at -20°C or below and can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Recommended antibody dilutions:
Working concentration for specific applications should be determined by the end user. The appropriate concentration may be affected by many factors, such as secondary antibody affinity, the sensitivity of the method of detection, temperature, incubation duration etc. The suitability of this antibody for applications other than those listed below has not been determined.
ELISA: 1:1000-1:10000
Western blot: 1:500-1:2000
Dot blot: 1:500-1:2000
Other applications: user-optimized
Application data：
Western blot analysis of several his-tag proteins (lane2-lane6), using similar product of a well-known manufacturer ( Figure. A) or our product GSB0001 (Figure. B). Lane 1 is added with 293T cell lysate. The antibody dilutions are both 1:2000.
Companion products：
#GSB0002 Anti-His(HRP)，McAb，mouse
#GSB0003 Anti-His(biotin)，McAb，mouse
