GSB0001

Anti-His, McAb, mouse

50μg/100μg/200μg/500μg/1000μg

 

Catalog number

Unit size

List price(CNY)

1

GSB0001-50

50μg

1700

2

GSB0001-100

100μg

3200

3

GSB0001-200

200μg

6000

4

GSB0001-500

500μg

12000

5

GSB0001-1000

1000μg

20000

Description

GSB0001 specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, such as ELISA, Western blot and Dot blot.

As an immunogen, 6×His-tag is poorly immunogenic, so it needs to be conjugated to KLH or some other carriers. GSB0001 is produced from the hybridoma which is fusion of Sp2/0-Ag14 mouse myeloma and B-lymphocytes obtained from mouse. After hundreds of selection cycles, our researchers successfully isolated an antibody against His-tag with high specificity and sensitivity. 

Key features

Source:                                                                

This monoclonal antibody GSB0001 is produced by mice immunizing with synthetic peptide HHHHHH coupled to KLH.

Isotype: Mouse IgG

Concentration: 1mg/mL

Applications: ELISAWestern blotDot blot and other kinds of immunoassays

Storage:                                                                

This antibody is stable in frozen form if stored at -20°C or below and can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Recommended antibody dilutions:

Working concentration for specific applications should be determined by the end user. The appropriate concentration may be affected by many factors, such as secondary antibody affinity, the sensitivity of the method of detection, temperature, incubation duration etc. The suitability of this antibody for applications other than those listed below has not been determined.

ELISA: 1:1000-1:10000
Western blot: 1:500-1:2000
Dot blot: 1:500-1:2000
Other applications: user-optimized

Application data


Western blot analysis of several his-tag proteins (lane2-lane6), using similar product of a well-known manufacturer ( Figure. A) or our product GSB0001 (Figure. B). Lane 1 is added with 293T cell lysate. The antibody dilutions are both 1:2000.


Companion products

#GSB0002  Anti-His(HRP)McAbmouse

#GSB0003  Anti-His(biotin)McAbmouse