rProtein A resin

#GSB1004

Recombinant Protein A Beads

1mL

 

Catalog number

Unit size

List price(CNY)

1

GSB1004

1mL

200

Description

Recombinant Protein A beads is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein A ligand is coupled to 6% highly cross-linked agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A beads is greater than 30 mg rabbit IgG/ml settled beads. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
    Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.

Key features

Species: Recombinant Protein A expressed in E.coli.

Storage: Store at 4°C, Do NOT freeze. 1mL settled beads (2 ml 50% slurry)

Note: High temperature heating is not recommended. The agarose melts above 65°C.

Characteristics:

Ligand

Recombinant Streptococcal protein A expressed in E. coli

Number of IgG binding sites per ligand

5

MW of ligand

Approximately 34 kDa

PI of ligand

5.17

Degree of substitution

Approximately 4 mg protein A/mL wet gel

Static binding capacity

>30 mg IgG/mL wet gel

Matrix spherical

agarose, 6% cross-linked

Average particle size

90 μm (45-165 μm)

Storage solution

20% ethanol

Storage & Stability

12 months when stored unopened at 2-8

Application data:


Figure1 Lane1: human serum; Lane2:Flow through; Lane3: wash; Lane4: elute;


Figure2 Lane1: human serum; Lane2: Flow through; Lane3: wash; Lane4: elute;

Figure 1 shows purification results of human serum by rProtein A beads of competitor. Figure 2 shows purification results of human serum by rProtein A beads of ZonHon Biopharma.