GSB5002

NCI-H2452 [H2452]

Organism: Homo sapiens, human/ Tissue: lung / Disease: mesothelioma

 

Catalog number

Unit size

List price(CNY)

1

GSB5002

-

1000

General information

Product Format: Culturing in 25cm2 Flask

Morphology: epithelial

Culture Properties: adherent

Biosafety Level: 1

Gender: male

Characteristics

Derivation: The line was established in November 1990 from a non-smoker with mesothelioma

Culture method

Complete Growth Medium:

The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing:

Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

1.      Remove and discard culture medium.

2.      Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.      Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.      Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5.      Add appropriate aliquots of the cell suspension to new culture vessels.

6.      Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Sekido Y, et al. Neurofibromatosis type 2 (NF2) gene is somatically mutated in mesothelioma but not in lung cancer. Cancer Res. 55: 1227-1231, 1995. PubMed: 7882313

Penno MB, et al. High CD44 Expression on Human Mesotheliomas Mediates Association with Hyaluronan. Cancer J. Sci. Am. 1: 196-203, 1995.